Gas Chromatography-Mass Spectrometric Determination of Sarin Exposures in Human Serum by Fluoride Reactivation Method

The fluoride ion reactivation method was used to determine sarin exposures in human serum using the gas chromatography-mass spectrometry technique. The reaction and extraction conditions were systematically investigated and optimized to regenerate the maximum amount of sarin. The highest reactivation efficiency was 86%, which was calculated from the recovery of butyrylcholinesterase activity. For regenerated sarin over the inhibition of butyrylcholinesterase activity, the linear calibration curve ranged from 6% to 85% and the limit of detection was 4%. No interference of human serum sample was found in this method. It can be used for the retrospective detection of sarin exposures.