Selection and Identification of Differential Proteins of Liver Revealed with Proteomics in Achatina Fulica Under Induction of Cadmium

In comparison with direct lysis, acetone/trichloroacetic acid (TCA) precipitation was selected to optimize and separate whole protein from the liver tissue of Achatina fulica (AF), and approximately 600 protein spots were obtained in gel. Using the cabbage exposed to 0.05 ppm CdCl2 as AF food, the liver was selected to reveal the stress proteins in the AF exposed to acute cadmium toxicity. Fourteen of differential protein spots in AF liver were obtained by proteomics, and then seven of these spots were further identified by both peptide mass fingerprint and database search, which indicated that these proteins in part were identified to be heat shock protein, methyltransferase, ABC transporter, molybdate transporter subunit, and phosphoglycerate mutase. We suggest that these significant proteins are connected with metabolism pathway of cadmium, biomarkers for monitoring the contamination level of the cadmium, and evaluating the crisis level of both food and soil.