Determination of Selenium Species in Burdock and Panax Notoginseng Using Ultrasonic Assistant Extraction Combined with High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry

A HPLC-ICP-MS method for the analysis of selenocystine (SeCys2), selenite [Se(IV)], selenomethionine (SeMet) and selenate [Se(VI)] was developed. In the experiment, chromatographic separation was performed within 8 min with 8 mM citric acid solution (pH 5) as mobile phase. The potentially interference of 40Ar40Ar+ to 80Se+ at the selenium masses of m/z 80 was reduced by CH4 as a reactive cell gas in the dynamic reaction cell. The selenium with different species was extracted using ultrasonic extraction system with a mixture of protease K and lipase. The limits of detection for SeCys2, Se(IV), SeMet and Se(VI) were 0.088, 0.033, 0.30 and 0.17 ng mL−1, respectively. The correlation coefficients were greater than 0.9995. The recoveries were in the range of 95.1%–114.6%. The developed method was successfully applied for the determination of selenium species in traditional Chinese medicine of Burdock and Panax notoginseng.

Graphical abstractA method for determination of selenium species in Burdock and Panax Notoginseng was developed using ultrasonic assistant extraction (USAE) combined with HPLC-ICP-MS. The potentially interference of 40Ar40Ar+ to 80Se+ was reduced by CH4 as a reactive cell gas in the dynamic reaction cell.Figure optionsDownload full-size imageDownload as PowerPoint slide