Analysis of Urine Peptidome and Post-translation Modifications by Nano Liquid Chromatography-High Resolution Tandem Mass Spectrometry

Proteomics is an emerging field branching from proteomics that targets endogenous peptides of a whole organism or a subsystem. Many peptides in body fluids including urine are biomarkers with higher clinical sensitivity and specificity. In this study, we separated and enriched these peptides in human urine by LaPO4-graphene oxide composite nanomaterial, then identified and analyzed them by nano liquid chromatography-high resolution tandem mass spectrometry. In single urine sample, we identified 790 peptides which belonged to 123 proteins and some post-translation modifications including oxidation, phosphorylation, deamidated etc. There existed a series of peptide ladders in urine peptidomic. Finally, the string analysis at protein level revealed strong interaction among these proteins to which the identified peptides belong. This method can provide support for finding the biomarkers of disease in urine.

Graphical abstractHere, we separated and enriched the low abundance peptides in human urine by LaGM composites, then identified and analyzed by nanoLC-MS/MS. We identified 790 peptides arised from 123 proteins and PTMs. Finally, the analysis at protein level revealed strong interaction among these proteins. This method can provide support for urine peptidomics analysis.Figure optionsDownload full-size imageDownload as PowerPoint slide