Development of Integrated Microfluidic Magnetic Bioprocessor for Multi-biomarker Detection

By integrated magnetic sensors and microfluidic system, a microfluidic magnetic bioprocessor was developed for the rapid detection of multi-target biomarkers. Three digestive system tumor makers, α-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9) were simultaneously detected in immune reactions in the microchannel as a model system to evaluate this multi-target detection system performance. The experiment parameters including injecting flow velocity, immune reaction time and rinsing rate for each step were optimized. The standard curves were established for three tumor markers detection in serum samples, with the detection limits of 0.1 ng mL−1 (CEA), 0.1 ng mL−1 (AFP), 30 U mL−1 (CA19-9) and the dynamic ranges spanning four orders of magnitude. Multi-biomarker analysis could be completed within 30 min, and good linear relations were obtained between the results of magnetic bioprocessor and ELISA method, otherwise, the former had the advantages such as fast detection and high sensitivity.

Graphical abstractA microfluidic bioprocessor integrated a magnetic tunnel junction (MTJ) device and a microfluidic cartridge has been developed for rapid immunodetection of multi-targets. Three tumor makers (AFP, CEA, CA19-9) were tested simultaneously with the dynamic ranges spanning four orders of magnitude and the advantage of fast detection and high sensitivity.Figure optionsDownload full-size imageDownload as PowerPoint slide