Fluorescent Immunoassay of Fluoranthene in Water Samples with CdTe Nanoparticles Labeled with Anti-fluorethene-antibody

Detecting and analyzing the trace-level polycyclic aromatic hydrocarbons (PAHs) in the field of environmental monitoring is one of the research hotspots. Cadmium telluride (CdTe) was combined with anti-fluoranthene antibody in fluorescent immunoassay assay. We found that the labeled compounds had a good dispersion and stability and could enhance the fluorescence intensity. The direct competitive fluorescent immunoassay with CdTe–anti-fluoranthene antibody was developed to detect fluoranthene in water sample in the environment. The result showed that fluoranthene could be determined in the concentration range of 10−1 to 103 μg/L with a correlation coefficient of 0.9983, a sensitivity of 12.4 μg L−1 to IC50, and a detection limit of 13.1 ng L−1 to IC20. The recovery was between 95.1% and 111.0%, and the relative standard deviation was less than 9%. The method is accurate and reliable and meets the requirements for detecting trace environmental hormone pollutants.