Determination of Trace Chlorophenols Endocrine Disrupting Chemicals in Water Sample Using [Bmim]BF4-NaH2PO4 Aqueous Two-Phase Extraction System Coupled with High Performance Liquid Chromatography

Determination of trace endocrine-disrupting chemicals such as chlorophenols in a water sample was carried out by utilizing ionic liquid aqueous two-phase extraction systems coupled with high-performance liquid chromatography (HPLC). In our study, the target analytes were 2,4-dichlorophenol (2,4-DCP), 2,6-dichlorophenol (2,6-DCP) and 4-chlorophenol (4-CP). The effects of salt concentration, pH value of aqueous phase, extraction time and the amount of ionic liquid on extraction efficiency were investigated. The highest extraction efficiency for these three target analytes were obtained as the concentrations of NaH2PO4 aqueous, pH value, extraction time and the amount of ionic liquid were 0.5 g mL−1, 4.0, 4 min and 2 mL, respectively. Ionic liquid phase was analyzed by HPLC directly with the detection limits of 2, 11 and 5 μg L−1 (S/N = 3) for 2,4-DCP, 2,6-DCP and 4-CP, respectively. Good recovery results (90.2%–107.0%) with relative standard deviation ranging from 1.2% to 5.2% (n = 5) were obtained when this method was applied to determine these three chlorophenols in tap water, lake water, waste water of chemical industry.