| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1182787 | Chinese Journal of Analytical Chemistry | 2013 | 6 Pages |
To study the impact of different temperatures on the growth and proliferation of myoblasts, specific electric fields were loaded on indium tin oxide (ITO) glass chips to generate temperature profiles. In several zones with different temperatures (38, 39, 40 and 41 °C), microchambers with equal size were fabricated for the culture of mouse myoblasts. Impacts of temperatures on growth and proliferation of myoblasts were studied by stimulating cells for continuous 5 days (30 min/day). Microscopic observation of cell morphology and flow cytometric detection results showed that certain thermal stimulation could promote the proliferation of myoblasts. The number of cells was obviously increased after stimulation at 40 °C. For equal total stimulation time, short-term and multiple thermal stimulation could achieve better effect, and maximum proliferation index could reach 38.39.
Graphical abstractSpecific electric fields were loaded on ITO glass chips to generate temperature profiles to study their impact on the growth and proliferation of myoblasts. Cells were stimulated for continuous 5 days, and the results showed that certain thermal stimulation could promote the proliferation of myoblasts. Short-term and multiple thermal stimulation could achieve better effect.Figure optionsDownload full-size imageDownload as PowerPoint slide
