Quantification of Dechlorane Plus in Human Serum by Gas Chromatography Coupled with Negative Chemical Ionization Mass Spectrometry

A gas chromatography coupled with negative chemical ionization mass spectrometry (GC-NCI-MS) was developed for the determination of two dechlorane plus (DP) isomers in human serum sample. Target compound and serum lipid were extracted by liquid-liquid extraction. After the sulfuric acid treatment to remove extracted lipids from the samples, samples were further cleaned up with a silica/sulfuric acid column. Identification and quantification of DP were carried out by MS. The detection limit of instrument was 0.5–0.7 pg. The detection limit of the method was 15–20 pg per sample. Recoveries of DP spiked in human serum samples were in the range of 70.2%–85.7%. The developed method was further validated in human serum. DP concentrations were analyzed in 20 electronics dismantling workers and 20 reference humans; DP concentration in dismantling workers and reference humans were 7.8–465 ng g−1 lipid and 0.93–50.5 ng g−1 lipid, respectively.