Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1182845 | Chinese Journal of Analytical Chemistry | 2013 | 5 Pages |
Abstract
A novel DNAzyme catalytic amplification biosensing platform has been developed for the colorimetric detection of lead ions (Pb2+) by utilizing the catalytic cleavage of 8–17 DNAzyme and the catalytic redox of horseradish peroxidase (HRP)-mimicking DNAzyme. The effects of K+ concentration, pH and incubation time of the detection system were investigated. A good linear relationship between ABTS absorbance and Pb2+ concentration in the range of 5–100 nM with a detection limit of 3 nM was found. In addition, an outstanding selectivity of the sensor for Pb2+ against other environmentally related metal ions was observed.
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