| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1182961 | Chinese Journal of Analytical Chemistry | 2007 | 6 Pages |
The solid–liquid reaction method was used for the preparation of a new High Performance Liquid Chromatography (HPLC) baicalin-bonded stationary phase (BBSP) via amido linkage, by using γ-aminopropyl trimethoxy silane (TM-550) as a coupling reagent and baicalin as a ligand. The structure of the ligand was characterized by Fourier transform infrared spectrum, elemental analysis, thermal analysis and so on. The chromatographic performances of BBSP were preliminarily evaluated, and a related retention mechanism was discussed by using different solutes as probes. Meanwhile, a comparative study between BBSP and conventional octadecyl-bonded stationary phase (ODS) was also carried out under the same conditions. The results showed that the new stationary phase had a relatively weak reversed-phase chromatographic property. Besides hydrophobicity, BBSP could also provide various sites, such as, hydrogen-bonding, π-π, and n-π charge transfer interactions. Synergistic interactions improved the separation selectivity of BBSP for aromatic compounds and their positional isomers when compared with ODS. It could also be observed that the polar amido linkage and hydrophilic glucose residue could minimize the residual silanols effect and improve the separation of basic nitrogen-containing compounds. Additionally, the new packing had strong affinity to flavonoids because of their similar structures. BBSP could be used as an enriching column packing for natural organic flavonoids from medicine herbs.
