Preparation of Polymer-bonded Chiral Ligand Exchange Chromatographic Stationary Phase and Resolution of Racemates

A novel stationary phase was synthesized for chiral-ligand-exchange chromatography by treating the monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) (PGMA/EDMA) beads with L-hydroxyproline. The enantiomeric resolutions of some D,L-amino acids and α-hydroxy acids were achieved on the synthesized chiral ligand exchange chromatographic stationary phase using 0.2 M NaAc and 0.1 mM Cu(Ac)2 aqueous solution as mobile phase, with pH 5.2, and column temperatures 30 °C and 50 °C. The influence of the mobile phase pH, the temperature of column, the flow rate of eluent, and the sample concentration on the resolution of D,L-amino acids by ligand exchange chromatography were investigated for the optimization of the chromatographic conditions. The experimental results showed that five of the nine enantiomers could be baseline separated and the best value of enantioselectivity (α) was 2.32.