| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1183320 | Chinese Journal of Analytical Chemistry | 2007 | 4 Pages |
A method was presented for the simultaneous determination of testosterone, methyltestosterone, and progesterone in liquid cosmetics by coupling polymer monolith microextraction to high performance liquid chromatography with UV detection. A poly (methacrylic acid-co-ethylene glycol dimethacrylate) monolithic capillary column was selected as the extraction media, which showed high extraction capacity towards these compounds. To achieve optimum extraction performance, several parameters relating to polymer monolith microextraction were investigated, including extraction flow-rate and pH value, inorganic salt concentration, and organic phase content of the sample matrix. By simple dilution with phosphate solution and filtering, the sample solution could then be directly injected into the extraction device. The limits of detection of testosterone, methyltestosterone, and progesterone were calculated to be 2.3, 2.8 and 4.6 μg 1−1, respectively. Good linearity was achieved in the range of 10–1000 μg 1−1 with a linear coefficient r above 0.996. Excellent method reproducibility was found by intra- and inter-day precisions yielding the relative standard deviations of <7.7% and <7.5%, respectively. The recovery for them in real samples was between 83% and 119%.
