| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1183545 | Chinese Journal of Analytical Chemistry | 2007 | 5 Pages |
A novel method for the quantification of minor 3-nitrotyrosine in the NaNO2-H2O2-hemin-tyrosine system was established by micellar electrokinetic chromatography with on-column high-salt stacking. The optimum CE separation conditions were as follows: 36 mM Na2B4O7 as the running buffer contained 30 mM SDS at pH 9.3; injection time 30 s at pressure of 3.44 kPa; separation voltage 20 kV, a temperature of 25°C; and a sample matrix containing 100 mM of NaCl. Under CE conditions, the linear relationship range from 0.78 to 50 μM (r = 0.9953), and the limit of detection (LOD) was 0.1 μM (S/N = 3). The RSDs of migration time for inter- and intra-day were 1.6% and 2.8%, respectively. The recoveries were 95.2%–103.1% and the RSDs for inter- and intra-day were less than 3% at various spiked levels. This study provides a useful method for the direct analysis of minor 3-NT in biological samples. This method is simple, rapid, and sensitive.
