| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1183744 | Food Chemistry | 2017 | 6 Pages |
•A fluorescent aptasensor has been developed for detection of AFB1 in infant food.•TAMRA at the end of probe plays as a quencher in fluorescent aptasensor.•The signal increases with increasing the concentration of the AFB1.•The sensor provides rapid, sensitive, and detection of AFB1.•This method has the potential for AFB1 analysis in diverse foods.
A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5–100 ng/mL with a detection limit down to 1.6 ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%–106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.
