| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1183875 | Food Chemistry | 2014 | 7 Pages |
•Anti-glycinin monoclonal antibodies were produced based on soy-free mouse model.•A sandwich ELISA is developed based on Mab 6F2 for detection of soybean glycinin.•The assay shows high affinity and specificity in comparison with previous methods.•We analysed the content of glycinin in 469 soybean seeds and five soybean products.
This study performs a sandwich ELISA for detection of trace amounts of glycinin in soybean products. We designed a soy-free mouse model to produce anti-glycinin monoclonal antibodies with high affinity and specificity. Using the monoclonal antibody as coating antibody, with the rabbit anti-glycinin polyclonal antibody as a detected antibody, the established sandwich ELISA showed high specificity for glycinin with minimum cross-reactions with other soy proteins. The practical working range of the determination was 3–200 ng/mL with detection limit of 1.63 ng/mL. The regaining of glycinin in spiked soybean samples were between 93.8% and 103.3% with relative standard deviation less than 8.3% (intra-day) and 10.5% (inter-day). The developed assay was used in analysing 469 soybean samples and five soybean products under different processing. The assay provides a specific and sensitive method for screening of glycinin and allows for further investigation into hypersensitive mechanisms to soybean proteins.
