Novel multiresidue method for determination of pesticides in red wine using gas chromatography–mass spectrometry and solid phase extraction

•New GC–MS multiresidue method for detection of 25 pesticides in red wine.•New in SPE: methanol and water reduces anthocyanins interference.•Method is fast with high recoveries and good precision.•Applicable for most popular pesticides in grape/wine application.•In 32 wine samples 7 pesticides were present at high concentrations.

A new multiresidue method was developed for determination of 25 pesticide residues in red wine by gas chromatography coupled to mass spectrometry with a single run of 23.63 min. Samples were extracted from wine with solid phase extraction using Oasis HLB. Mixture of methanol and water was used for rinsing, while acetonitrile and n-hexane were used as elution solvents. Method was validated according to SANCO/12571/2013 criteria in wide linearity range (limit of quantification – 400 μg L−1). Limits of quantification (LOQ) were well below 10 μg L−1 for most pesticides and recoveries at 2 × LOQ and 10 × LOQ concentration levels were in range 70–120%. Precision, expressed as a relative standard deviation, was always under 14%. The method was applied to 32 red wine samples from Croatia. Pesticides were detected in 30 samples with a total of 15 pesticides found, 7 of which were at a high concentration.