Novel analytical procedure using a combination of hollow fiber supported liquid membrane and dispersive liquid–liquid microextraction for the determination of aflatoxins in soybean juice by high performance liquid chromatography – Fluorescence detector

•High sensitive analysis of AFLs in soybean juices samples at μg L−1 levels.•Hollow fiber coupled with dispersive liquid–liquid microextraction.•Extraction free of chlorinated solvents and less than 500 μL of another solvents.•Great potential for automation and application for other analytes and samples.

This study describes a combination between hollow fiber membrane and dispersive liquid–liquid microextraction for determination of aflatoxins in soybean juice by HPLC. The main advantage of this approach is the use of non-chlorinated solvent and small amounts of organic solvents. The optimum extraction conditions were 1-octanol as immobilized solvent; toluene and acetone at 1:5 ratio as extraction and disperser solvents (100 μL), NaCl at 2% of the sample volume and extraction time of 60 min. The optimal condition for the liquid desorption was 150 μL acetonitrile:water (50:50 v/v) and desorption time of 20 min. The linear range varied from 0.03 to 21 μg L−1, with R2 coefficients ranging from 0.9940 to 0.9995. The limits of detection and quantification ranged from 0.01 μg L−1 to 0.03 μg L−1 and from 0.03 μg L−1 to 0.1 μg L−1, respectively. Recovery tests ranged from 72% to 117% and accuracy between 12% and 18%.