Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

•DAOY cells have been analyzed by top-down LC-high resolution-MS proteomic platform.•New protein identifications in medulloblastoma cells are reported.•PTMs, isoforms and naturally occurring peptide fragments were identified.•Most of the identified proteins were connected in a biological interacting network.•The data contribute to the further molecular characterization of medulloblastoma.

The proteome of the DAOY medulloblastoma cell line has been investigated by an LC–MS top-down platform. This approach, unlike bottom-up ones, allows identifying proteins and peptides in their intact/native forms, disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that characterize DAOY cells.

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