Article ID Journal Published Year Pages File Type
1184499 Food Chemistry 2015 8 Pages PDF
Abstract

•Formation of multilayer o/w using the LbL method increases the stability within GIT.•Salt addition or enzymatic crosslinking could not prevent hydrolysis of lipids.•The rate of lipid digestion was faster in double-layer o/w than single-layer o/w.

In this study, membrane properties were modulated using layer-by-layer electrostatic depositioning in combination with salt and/or enzyme treatment to control the gastrointestinal fate of emulsified oils. Lipid droplets coated by a single-layer of biopolymers (gelatin) were prepared by high pressure homogenization. Lipid droplets coated by a double-layer of biopolymers (gelatin–pectin) were prepared by electrostatically depositing sugar beet pectin on the gelatin-coated droplets. Laccase was added to the double-layer emulsions to covalently crosslink the adsorbed pectin molecules, whereas sodium chloride was added to modulate interfacial properties through electrostatic screening effects. Non-cross-linked and cross-linked double-layer emulsions (with and without salt) were then passed through a simulated gastrointestinal tract (GIT) that included mouth, gastric and intestinal phases. Free fatty acid release profiles suggested that the stability of the emulsified droplets within the GIT played a more important role in determining the rate and extent of lipid digestion than the initial interfacial layer properties.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , ,