| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1184544 | EuPA Open Proteomics | 2015 | 7 Pages |
•2D RP/RP LC–MS/MS improves proteome and protein coverage relative to 1D analysis.•Sulforaphane does not influence global protein profile in LNCaP prostate cancer cells.•Global analysis identified the protein TRIAP1 in LNCaP cells.•TRIAP1 influences LNCaP proliferation and is a potential therapeutic target.
The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC–MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC–MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.
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