| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1185068 | Food Chemistry | 2016 | 9 Pages |
•Water soluble polysaccharides were extracted from Z. lotus fruit.•Box–Behnken design of RSM methodology was used for optimization of polysaccharides extraction conditions.•Macromolecular characterization and monosaccharide composition of polysaccharides extract were determined.•Polysaccharides from Z. lotus fruit (pulp and peel) exhibited significant antioxidant activity.
Response surface methodology using a Box-Behnken design was employed to optimize extraction temperature, extraction time and ratio of water to material to obtain a maximum polysaccharide yield with high uronic acid content and antioxidant property from edible Zizyphus lotus fruit. The optimal conditions were: extraction time of 3 h 15 min, extraction temperature of 91.2 °C and water to solid ratio of 39 mL/g. Under these conditions, the experimental extraction yield, uronic acid content and 2,2-diphenyl-1-picrylhydrazyl scavenging ability (IC50) were 18.88%, 41.89 and 0.518 mg/mL, respectively. Chemical analysis revealed that the extract was composed of 97.92% carbohydrate of which 41.89% is uronic acid. The extracted polysaccharides, with an average molecular weight of 2720 kDa, are composed of arabinose, rhamnose, glucose, fructose, galactose and xylose. Moreover, the polysaccharides exhibited a significant reducing power and anti-lipid peroxidation activities.
