| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1185108 | Food Chemistry | 2016 | 9 Pages |
•A LC-MS/MS method was developed to detect three sulfonamide potentiators in animal feed.•A dichloromethane extraction method was established.•The method was validated to have high recovery, sensitivity, and robustness.•The method was successfully applied to detect three sulfonamide potentiators in feed.
This study developed and validated a simple and reliable method for detecting and quantifying DVD, TMP and OMP in feed using dichloromethane extraction followed by HPLC-MS/MS. A matrix effect evaluation was performed using the post-extraction spiking method, and levels were less than ±15% in all three feeds with their corresponding concentrations. LOD and LOQ, CCα and CCβ were 20 μg kg−1 and 40 μg kg−1, 8.68–15.55 μg kg−1 and 10.61–18.92 μg kg−1 for all analytes, respectively. Calibration curves were linear for DVD, TMP and OMP with R2 ⩾ 0.990 and r ⩾ 0.995, respectively. Recoveries of low, medium and high concentrations using the proposed method ranged from 74.4 to 105.2%. Repeatability and within-laboratory reproducibility were <7.4% (RSD). The chosen seven factors had no a significant influence on robustness. The method showed good performance when it was applied to analyze other laboratory-prepared or actual feed samples.
