Article ID Journal Published Year Pages File Type
1185372 Food Chemistry 2010 6 Pages PDF
Abstract

The thermal stability of recombinant mannose-specific banana lectin (rBanLec), as well as its stability under conditions of simulated gastro-intestinal fluid (SGF), was investigated. rBanLec was heterologously produced in Escherichia coli. Molecular mass of rBanLec, assessed by ESI-TOF mass spectrometry, was 15972.2 Da. Thermodynamic parameters for rBanLec denaturation, obtained by differential scanning calorimetry (DSC), revealed a transition maximum temperature (Tm) of 60.8 °C, calorimetric enthalpy (Hcal) of 136.17 kcal/mol and van’t Hoff enthalpy (HVH) of 50.27 kcal/mol. rBanLec was stable following an incubation for 2 h in SGF, and then for 1 h, in the simulated intestinal fluid (SIF). Intact primary structure, biological and immunological reactivity of rBanLec were all preserved following treatment under SGF and SIF conditions. In conclusion, rBanLec is a good candidate for the novel bioadhesive lectin-based drug delivery systems to the gastro-intestinal tract (GIT).

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Physical Sciences and Engineering Chemistry Analytical Chemistry
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