Article ID Journal Published Year Pages File Type
1186066 Food Chemistry 2012 4 Pages PDF
Abstract

Colon cancer is one of the leading causes of death and a major public health problem in western countries. We examined the isoflavone content of 70% ethanol extract of soy germ (SG) and its apoptotic effect in HT-29 colon cancer cells. Our results showed that the major isoflavones of the SG extract were daidzein and genistein, and it effectively induced apoptosis in HT-29 cancer cells. In addition, nuclear factor-kappa B (NF-κB) expression was reduced in cells treated with the SG extract, which reduced cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) expression. These combined effects ultimately resulted in apoptosis via caspase-3 activation. In addition, daidzein and genistein, the major isoflavones of SG extract, also exerted the apoptotic effect against HT-29 cancer cells. These results suggest that the inhibition of NF-κB expression is the most important factor in the induction of apoptosis in HT-29 cells treated with the SG extract.

► Daidzein and genistein was the major isoflavone of a soy germ extract. ► They contributed to the apoptotic effect of a soy germ extract in HT-29 cells. ► The soy germ extract may be used as supplementary strategy for cancer prevention.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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