Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1186136 | Food Chemistry | 2009 | 5 Pages |
Abstract
Gastrointestinal (GI) mimic and organic solvent extracts of whole soybean powder (WSP), soy protein concentrate (SPC), and soy protein isolate (SPI) as well as soy isoflavone concentrate (SIC) were analysed for total phenols; quinone reductase (QR) induction in hepa1c1c7 cells; antioxidant scavenging of DPPH, HOCl, ONOOâ, and O2-; and total antioxidant capacity via FRAP and ORAC assays. GI extracts of all the soy products had higher concentrations of total phenols than from acidified methanol (MeOH) but lower antioxidant potency. The MeOH extract of SPC was most potent in quenching HOCl and ONOOâ and increasing FRAP and ORAC, but did not induce QR. Despite weak antioxidant activity, hexane (HX) extracts induced QR more than GI and MeOH extracts with WSPÂ >Â SPCÂ >Â SPIÂ >Â IC. Soy extracts were ineffective scavengers of DPPH and O2-. Thus, extraction methods markedly affect the antioxidant profile and QR induction capacity of soy products.
Keywords
SPCGAENBTFRAPWSPAAPHSPISiCDHR 123ORACLSDDPPHDMEM2,2′-azobis (2-amidinopropane) dihydrochloride2,2-diphenyl-1-picrylhydrazylnitroblue tetrazoliumAntioxidantExtractiongallic acid equivalentsTrolox EquivalentsSoy protein isolateleast significant differenceGastrointestinaldihydrorhodamine 123Soyoxygen radical absorbance capacityMeOHhexaneferric reducing antioxidant powerSoy protein concentrateQuinone reductase
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bradley W. Bolling, Jeffrey B. Blumberg, C.-Y. Oliver Chen,