Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1186173 | Food Chemistry | 2010 | 9 Pages |
This study describes the in vitro metabolism, by the gastrointestinal tract, together with the biological activity of the herbal tea obtained from Plectranthus barbatus leaves. The results showed that there was no appreciable degradation and that the activity was kept constant after gastric juice digestion. In the presence of pancreatic juice, degradations varied from negligible, in the case of flavonoid glucuronides, to almost complete, in the case of the abietane diterpenoid. The digested decoction contained only 50% of its initial biological activity, after pancreatic digestion. The action of Caco-2 cells on the extract revealed that neither rosmarinic acid, the main compound of the extract, nor the other components present in minute quantities were metabolised by the intact cells. Rosmarinic acid could be found inside Caco-2 cells although in trace amount. Glucoronidase from Escherichia coli, a gut bacterium, was able to hydrolyse the flavonoid derivatives, thus the aglycones were formed and permeate the Caco-2 cells.