Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1187375 | Food Chemistry | 2009 | 6 Pages |
Abstract
A visceral excitatory neuropeptide with a molecular weight of 1563Â Da was isolated from the brain extracts of cloudy dogfish (Scyliorhinus torazame) discarded as a fishery by-product. Fish hindgut was employed to carry out physiological assay for its visceral excitation. During consecutive purification using C18 cartridge and high-performance liquid chromatography (HPLC), a neuropeptide (DF-2) was isolated from neuronal cell extracts of dogfish brain tissue and clearly exhibited potential to exert visceral excitatory effect on hindgut of dogfish. The primary structure of DF-2 established by ESI-Q-TOF tandem mass was LESLVYEQLWPWamide. The results of database search provided evidence for this peptide sequence to be novel. In visceral excitatory assay using myography, the threshold concentration of DF-2 required for the changes in spontaneous contraction of cloudy dogfish hindgut was found to be 10â6Â M. Therefore, this study suggests that DF-2 isolated from brain of elasmobranches could be one of the sequences, which regulates spontaneous visceral contractions in dogfish digestive duct.
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Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Young-Sook Cho, Won-Kyo Jung, Sang-Hoon Lee, Eresha Mendis, Se-Kwon Kim,