Spectroscopic investigation of structure-breakers and structure-makers on ornithine carbamoyltransferase

It is well known that the cosolutes can affect the solvent properties and therefore influence the environment in which biostructures are immersed. Structure-breakers or “chaotrope” solutes do not stabilize biomolecules, whereas structure-makers or “kosmotrope” solutes are capable to create strong bonds with water, so creating good conditions for avoiding denaturation processes. The effect of different additives and temperature on ornithine carbamoyltransferase has been carried out using techniques such as the measurement of the enzymatic activity and UV-VIS spectroscopy.