| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1188140 | Food Chemistry | 2012 | 6 Pages |
In the present study, we have investigated the influence of low concentrations of polyphenols (5 μM) on histone acetyltransferase (HAT) and histone deacetylase (HDAC) activity, a monocyte cell system related to the inflammatory process. We selected gallic acid, ellagic acid, oenothein B, valoneic acid dilactone and penta-O-galloyl-β-d-glucose, which are known to affect inflammatory responses, such as IL-6 secretion and/or NF-κB down-regulation and polyphenol metabolites (urolithins A, B and C). TNF-α stimulation resulted in a reduction of THP-1 cell viability by 28.9%, reduced HDAC activity from 25.2 to 13.5 pmol/μg protein and increased HAT activity from 24.0 to 45.5 pmol/μg protein. The coincubation with ellagic acid and oenothein B restored the viability and reversed the effect of TNF-α on HAT and HDAC activities. Urolithins B and C, gallic acid and penta-O-galloyl-β-d-glucose only showed significant reductions of HAT activity, by 40–50%. Our results prove that polyphenols act as epigenetic modulators.
► We investigated the influence of polyphenols on HAT and HDAC activity in THP-1 cell. ► Ellagic acid showed the strongest effects on both enzymes activity. ► Our results prove that polyphenols act as epigenetic modulators.
