Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma)

Acid-soluble collagen (ASC) was extracted from the skin of walleye pollock (Theragra chalcogramma) and partially characterized. It exhibited a maximum absorbance at 220 nm, but little absorbance near to 280 nm. Amino acid composition and SDS-PAGE suggested that the collagen might be classified as type I collagen. Moreover, FTIR investigations showed the existence of helical arrangements of collagen. The denaturation temperature (Td) and shrinkage temperature (Ts) were 24.6 °C and 47 °C, respectively, both lower than those of mammalian collagens. However, Td of walleye pollock skin collagen was higher than that of cod skin collagen reported previously. These results indicate that walleye pollock skin is a potential source of collagen and provide the theoretical basis for further research.