Raspberry seed extract effect on the ferroxidase activity of ceruloplasmin isolated from plasma

In this study, the effect of the raspberry seed extract (RSE) on the ferroxidase activity of ceruloplasmin (Cp) isolated from healthy male subject serum was analyzed. The ferroxidase activity of Cp was determined by spectrophotometry using Fe(II) – histidine complex and ferrozine as a chromogenic reagent. The ferroxidase activity of ceruloplasmin was demonstrated in dose-dependent way within the range 22–66 μg/ml. The effect of RSE on Fe(II) concentration, measured as the decrease of Fe(II) concentration in samples and expressed as ΔFe(II), was found to be a dose-dependent within the range 1.20–51.56 μg dm/ml. The ferroxidase activity of Cp was influenced by the RSE within its studied range. However, the addition of the highest concentration of RSE (51.56 μg dm/ml) to the sample containing the highest level of Cp (66 μg/ml) did not affect its ferroxidase activity. It may be suggested that the competition for Fe(II) as the substrate limits the effect of RSE on Cp activity and causes no further changes in Fe(II) elimination.