Article ID Journal Published Year Pages File Type
1189372 Food Chemistry 2006 9 Pages PDF
Abstract

Feruloyl oligosaccharides production from wheat bran insoluble dietary fibre was carried out using xylanases from Bacillus subtilis. Response surface methodology (RSM), based on a five level, five variable central composite rotatable design (CCRD), was employed to obtain the best possible combination of reaction temperature, pH, time, enzyme concentration and substrate (wheat bran insoluble dietary fibre) concentration for maximum feruloyl oligosaccharides production. The optimum reaction conditions were as follows:temperature, 42 °C; pH, 5.2; reaction time, 35 h; enzyme concentration, 4.8 g/l and substrate concentration, 120 g/l. Under these conditions, the experimental yield was 1.55 mM, which is well matched with the predictive yield.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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