Production of feruloyl oligosaccharides from wheat bran insoluble dietary fibre by xylanases from Bacillus subtilis

Feruloyl oligosaccharides production from wheat bran insoluble dietary fibre was carried out using xylanases from Bacillus subtilis. Response surface methodology (RSM), based on a five level, five variable central composite rotatable design (CCRD), was employed to obtain the best possible combination of reaction temperature, pH, time, enzyme concentration and substrate (wheat bran insoluble dietary fibre) concentration for maximum feruloyl oligosaccharides production. The optimum reaction conditions were as follows:temperature, 42 °C; pH, 5.2; reaction time, 35 h; enzyme concentration, 4.8 g/l and substrate concentration, 120 g/l. Under these conditions, the experimental yield was 1.55 mM, which is well matched with the predictive yield.