| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1189457 | Food Chemistry | 2011 | 6 Pages |
An assay for the measurement of antiradical activity profiles of free radical scavengers (sometimes called antioxidants) in plant extracts was optimised on a modular instrument to obtain rapid and precise results. The first step of a run was separation by RPHPLC recorded with a direct absorbance detector, followed by a post-column reaction with a radical form of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+). Then, the effect of colour bleaching of ABTS+ was measured on a second absorbance detector (indirect detection). A comparison of the two signals distinguished the analytes with antiradical activity.Improvements in technical features, namely the minimisation of reaction volume and the use of precise high-pressure pumps enabled both the detector signals to be precisely matched without significant loss of separation efficiency, even in complex chromatograms.
