Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1189931 | Food Chemistry | 2011 | 4 Pages |
Abstract
This paper describes an easy, small-scale method of sample preparation followed by a reversed-phase (RP) high-performance liquid chromatography (HPLC) for quantifying canthaxanthin (CX) in egg yolk. The egg yolk extract including CX was purified by using a normal-phase InterSep®-CN mini-syringe-column as a solid-phase extraction column. HPLC was performed on a C8 column with an isocratic mobile phase and diode array detector. The proposed method was validated by the analyses of spiked egg yolk samples; resulting recoveries (⩾94.5%; RSD ⩽ 2.8%), analytical total time (<1 h/sample), and limit of quantitation (0.5 μg/g). The decision limit and detection capability were 25.04 and 25.07 μg/g, respectively.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Naoto Furusawa,