Article ID Journal Published Year Pages File Type
1190011 Food Chemistry 2008 6 Pages PDF
Abstract

In this paper, a nano-NiO modified carbon paste electrode in capillary zone electrophoresis with amperometric detection (CZE-AD) was firstly applied to the determination of carbohydrates. Effects of several important factors such as detection potential, the concentration of running buffer, separation voltage and injection time were investigated to acquire the optimum conditions. Under the selected optimum conditions, three carbohydrates: glucose, sucrose and fructose could be perfectly separated within 20 min. The relationships between peak current and concentration of three carbohydrates were linear over about 3 orders of magnitude with detection limits (S/N = 3) ranging from 3.0 × 10−7 to 6.0 × 10−7 mol L−1. The electrode was stable, and can be used for at least one week. The proposed method has been successfully applied to monitor carbohydrates in the real samples with satisfactory assay results.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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