Article ID Journal Published Year Pages File Type
1190867 Food Chemistry 2007 5 Pages PDF
Abstract

Polyphenol oxidase (PPO) was extracted from Anamur banana, grown in Turkey, and its characteristics were studied. The optimum temperature for banana PPO activity was found to be 30 °C. The pH-activity optimum was 7.0. From the thermal inactivation studies, in the range 60–75 °C, the half-life values of the enzyme ranged from 7.3 to 85.6 min. The activation energy (Ea) and Z values were calculated to be 155 kJ mol−1 and 14.2 °C, respectively. Km and Vmax values were 8.5 mM and 0.754 OD410 min−1, respectively. Of the inhibitors tested, ascorbic acid and sodium metabisulphite were the most effective.

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Physical Sciences and Engineering Chemistry Analytical Chemistry
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