| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1191613 | Food Chemistry | 2007 | 8 Pages |
The idea of preparing the surface of Fe3O4 layer preadsorbed on the “spherical polyelectrolyte brush” via layer-by-layer self-assembly approach receives special relevance in enzyme technology. “Spherical polyelectrolyte brush” of core–shell structure were prepared by grafting poly(sodium 4-styrenesulphonate) (PSStNa) from SiO2 nanoparticles via the surface-initiated atom transfer radical polymerization strategy. The silica-coated (PEI/Fe3O4)n support presents a very simple, mild, and time-saving process for enzyme immobilization. The kinetics of a model reaction catalyzed by the immobilized pectinase was finally investigated by the Michaelis–Menten equation. These particles, premodified with the layer of magnetic nanoparticles to impart a magnetic property and subsequently coated with polyelectrolyte multilayer, were repeatedly used as catalysts following their rapid and easy separation with a magnet. Besides simplicity and versatility, the ease of enzyme regeneration constitutes an additional benefit of this approach.
