Article ID Journal Published Year Pages File Type
1191759 Food Chemistry 2007 10 Pages PDF
Abstract

The effects of ethanolic extract of sesame (Sesamum indicum L.) coat (EESC) on oxidation of low-density lipoprotein (LDL) and production of nitric oxide in macrophages were investigated. The results show that EESC in the range of 0.01–0.8 mg/ml markedly inhibited copper-induced LDL oxidation and H2O2-induced cell damage. In addition, EESC at 0.1 mg/ml not only enhanced glutathione (GSH) levels, but also increased activity of GSH peroxidase, GSH reductase, GSH transferase and catalase by 3.32-, 3.39-, 1.60- and 2.26-fold compared to the control, respectively. Moreover, EESC showed dose-dependent inhibitory effects on nitric oxide production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages and in sodium nitroprusside (SNP), a NO donor. The generation of prostaglandin E2 (PGE2) in stimulated macrophages was also reduced by EESC. The induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) proteins in activated macrophages was inhibited by EESC. In addition, the levels of iNOS and COX-2 mRNA in activated macrophages were decreased by EESC when determined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Furthermore, LPS-induced degradation of IκB-α was down regulated by EESC, indicating that EESC prevented LPS-induced NF-κB activation. These results implied that EESC could exhibit a protective action on biomolecules and generation of inflammatory mediators in vitro.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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