Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1192299 | International Journal of Mass Spectrometry | 2011 | 7 Pages |
Abstract
ⶠFormation of chitooligosaccharide-chitinase non-covalent complexes in the mass range of 55 kDa was dissected by high resolution mass spectrometry. ⶠChemical composition of bound and free chitooligosaccharide species and their products in complex mixtures was determined by FT-ICR MS at 9.4 T. ⶠWild-type chitinase B is shown to undergo with chitooligosaccharides four interaction/reaction pathways: (1) hydrolysis, (2) non-productive binding, (3) transglycosylation, and (4) lactone formation. ⶠSite-directed mutation D142N in chitinase B is shown to largely enhance the transglycosylation process. ⶠSite-directed mutagenesis E144Q in chitinase B on the binding specifity to chitooligosaccharides is changed and the enzymatic activity abolished.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
F. Henning Cederkvist, Michael Mormann, Martin Froesch, Vincent G.H. Eijsink, Morten Sørlie, Jasna Peter-KataliniÄ,