| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1192871 | International Journal of Mass Spectrometry | 2011 | 6 Pages |
Transmission mode desorption electrospray ionization is coupled with ultraviolet photodissociation at 193 nm for the analysis of protein digests. SEQUEST is utilized for data interpretation and database searches. Comparative results are presented for transmission mode desorption electrospray ionization (TM-DESI)-MS/collision induced dissociation (CID) and TM-DESI-MS/ultraviolet photodissociation (UVPD) analyses of five proteolyzed model proteins ranging in molecular weight from 8.5 kDa (ubiquitin) to 66 kDa (bovine serum albumin, BSA). In some cases TM-DESI/UVPD yielded greater confidence in database correlation scores for peptides and comparable protein identification compared to TM-DESI-MS/CID due to the production of an extensive array of sequence ions and the ability to detect low m/z terminal sequence ions and immonium ions.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (137 K)Download as PowerPoint slideHighlights► Transmission mode DESI was utilized for a proteomics application. ► Confident protein identification is achieved through sequencing by tandem mass spectrometry. ► Ultraviolet photodissociation at 193 nm is compared to collision induced dissociation.
