Two-dimensional FT-ICR/MS with IRMPD as fragmentation mode

In 1988, Gäumann et al. introduced a pulse sequence for two-dimensional FT-ICR/MS correlating parent ions and fragment ions without the need for ion isolation. The improvement in computer technology makes this pulse sequence analytically useful in order to obtain structural information on complex samples. The pulse sequence can be applied to all cyclotron radius-dependent fragmentation modes, including gas-free fragmentation modes like IRMPD, which do not affect sensitivity and resolving power like the pulsing of a gas into the ICR cell does. This study shows the feasibility of 2D FT-ICR/MS and lays the groundwork to turn this method into a viable analytical tool.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (256 K)Download as PowerPoint slideResearch highlights▶ 2D FT-ICR/MS adapts to digital instruments with gas-free fragmentation. ▶ 2D mass spectra show a good correspondence with theory and MS/MS spectra. ▶ 2D mass spectra of complex samples are sensitive over a large dynamic range. ▶ Techniques adapted from 2D NMR can turn 2D FT-ICR/MS into a viable analytical tool.