Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1193544 | International Journal of Mass Spectrometry | 2012 | 8 Pages |
Abstract
⺠A sensitive, specific, and high throughput method was developed and validated for the quantitation of free maytansinoid DM4 in cynomolgus monkey and Sprague Dawley rat plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). ⺠The quantitation range of the method was 0.500 - 100 ng/mL with a lower limit of quantitation of 0.500 ng/mL. ⺠The method was validated in monkey and rat plasma per FDA guidelinesThe validated method was employed to monitor the free DM4 levels in plasma in the IND-enabling toxicology studies of antitumor agent DM4 conjugated hu-anti-Cripto MAb B3F6 (B3F6-DM4).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dong Wei, Michael Sullivan, Orlando Espinosa, Liyu Yang,