An in-vacuum, pixelated detection system for mass spectrometric analysis and imaging of macromolecules

The analytical capabilities of a Timepix detection system for mass spectrometry (imaging) are extended by the application of additional acceleration fields. Electrically biasing the detection system at a high potential delivers effective ion acceleration energies of 15/11 keV for positive/negative ion mode matrix-assisted laser desorption ionization. This is comparable to the acceleration/extraction fields in commercially available time-of-flight mass spectrometers. This high voltage detector system enables measurements in both ion polarities and more sensitive analysis of higher molecular mass ions. The technical implementation of this system and first results in positive and negative ion mode are presented. The high voltage enhanced analytical capabilities of the Timepix detection system are demonstrated in the mass range up to 100 kDa.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (400 K)Download as PowerPoint slideHighlights► Highly parallel, pixelated detection system for positive and negative ion mode MS(I). ► In-vacuum/ultra-high vacuum-compatible mounting of pixelated detection system. ► Capability to detect higher mass intact proteins in both polarities. ► Protein detection in the mass range up to 100 kDa.