Enhanced Ion Signals in Desorption Electrospray Ionization Using Surfactant Spray Solutions

Solvent optimization is an important procedure in desorption electrospray ionization (DESI) and in this study the effects of solvent surface tension are explored. Data are presented for methanol/water/surfactant solvent systems, which show increases in ion signals of more than an order of magnitude when low concentrations of surfactants are added to the standard methanol/water (1:1) spray solvent. Examples of analytes tested include food chemicals, peptides, pharmaceuticals, and drugs of abuse. The improvement in ion intensity is mainly attributed to the effect of surface tension in producing smaller spray droplets, which are shown to cover a larger surface area. Surfactant-containing spray solutions allowed extension of DESI-MS analysis to previously intractable analytes like melamine and highly hydrophobic compounds like the sudan dyes.

Graphical AbstractSurfactants modify solvent surface tension extending DESI analysis to both hydrophobic and hydrophilic compounds. Signal intensity increases because the smaller droplets cover a larger sample area.Figure optionsDownload full-size imageDownload high-quality image (136 K)Download as PowerPoint slide