| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1195203 | Journal of the American Society for Mass Spectrometry | 2009 | 8 Pages |
Using both automated nanospray and online liquid chromatography mass spectrometry LC-MS strategies, 99 proteins have been newly identified by top-down tandem mass spectrometry (MS/MS) in Methanosarcina acetivorans, the methanogen with the largest known genome [5.7 mega base pairs (Mb)] for an Archaeon. Because top-down MS/MS was used, 15 proteins were detected with mispredicted start sites along with an additional five from small open reading frames (SORFs). Beyond characterization of these more common discrepancies in genome annotation, one SORF resulted from a rare start codon (AUA) as the initiation site for translation of this protein. Also, a methylation on a 30S ribosomal protein (MA1259) was localized to Pro59–Val69, contrasting sharply from its homologue in Escherichia coli (rp S12) known to harbor an unusual β-thiomethylated aspartic acid residue.
Graphical AbstractAutomated top-down characterization of Methanosarcina acetivorans on a 12 T LTQ FT Ultra utilizes high-resolution data to unambiguously discover unannotated proteins and new PTMs.Figure optionsDownload full-size imageDownload high-quality image (214 K)Download as PowerPoint slide
