An Experimental and Theoretical Investigation into the Hydrolysis of Dichloro(Ethylenediamine)Platinum(II) Via Electrospray Mass Spectrometry and Density Functional Theory

Dichloro(ethylenediamine)platinum(II), Pt(en)Cl2, was dissolved in H2O and D2O, and the resulting aqueous solutions were electrosprayed into a quadrupole ion-trap mass spectrometer. A series of major and minor ionic hydrolysis products were detected. These ions were then subjected to collision-induced dissociation. As an aid in interpreting the experimental results, density functional theory calculations were carried out. These computations permitted the structures and energetics associated with the hydrolysis products to be determined. An understanding of the hydrolysis of PtenCl2 and related coordination complexes is essential in the rational design of metal-based drugs.

Graphical AbstractAn investigation of the likely hydrolysis products of a cisplatin analog, Pt(en)Cl2, via electrospray and collision-induced dissociation mass spectrometry and density functional theory.Figure optionsDownload full-size imageDownload high-quality image (86 K)Download as PowerPoint slide