Ethylenediaminetetraacetic Acid (EDTA) as an Auxiliary Tool in the Electrospray Ionization Mass Spectrometry Analysis of Native and Derivatized β-Cyclodextrins, Maltoses, and Fructans Contaminated with Ca and/or Mg

The effect of Ca2+ (and Mg2+) and the disodium salt of ethylenediaminetetraacetic acid (EDTA), a well known Ca2+ (and Mg2+) chelating agent, on the volatilization/ionization of carbohydrates by using electrospray ionization mass spectrometry has been studied. Model compounds such as maltoses (maltose to maltoheptaose), β-cyclodextrins (β-cyclodextrin, methyl-β-cyclodextrin, heptakis(2,6-di-O-methyl)-β-cyclodextrin, heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin, and 2-hydroxypropyl-β-cyclodextrin) and fructans (sucrose, 1-ketose, nystose, and 1F-fructofuranosylnystose) were used.

Graphical AbstractAddition of EDTA to the carbohydrate aqueous solution quenches the Ca2+ (and Mg2+) cationizing capability in ESI because of its chelating property. The signals [M + Na]+ and/or [M + 2Na]2+ are restored.Figure optionsDownload full-size imageDownload high-quality image (67 K)Download as PowerPoint slide