The Use of Shift Reagents in Ion Mobility-Mass Spectrometry: Studies on the Complexation of an Active Pharmaceutical Ingredient with Polyethylene Glycol Excipients

Gas-phase ion mobility studies of mixtures containing polyethylene glycols (PEG) and an active pharmaceutical ingredient (API), lamivudine, have been carried out using electrospray ionization-ion mobility spectrometry-quadrupole-time-of-flight mass spectrometry (ESI-IMS-Q-TOF). In addition to protonated and cationized PEG oligomers, a series of high molecular weight ions were observed and identified as noncovalent complexes formed between lamivudine and PEG oligomers. The noncovalent complex ions were dissociated using collision induced dissociation (CID) after separation in the ion mobility drift tube to recover the protonated lamivudine free from interfering matrix ions and with a drift time associated with the precursor complex. The potential of PEG excipients to act as “shift reagents,” which enhance selectivity by moving the mass/mobility locus to an area of the spectrum away from interferences, is demonstrated for the analysis of lamivudine in a Combivir formulation containing PEG and lamivudine.

Graphical AbstractThe potential of PEG excipients to act as “shift reagents” in the ion mobility-mass spectrometry analysis of a formulated pharmaceutical containing the active lamivudine.Figure optionsDownload full-size imageDownload high-quality image (126 K)Download as PowerPoint slide