| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1196503 | Journal of the American Society for Mass Spectrometry | 2007 | 10 Pages |
Abstract
An on-line proteolysis system utilizing a triaxial electrospray probe was developed to aid localization of the hydrogen-bonding interaction sites in hydrogen/deuterium exchange-mass spectrometry (HDX-MS) studies of Aβ (1-40) fibrils. The probe allows delayed introduction of the organic solvent component needed for stable electrospray, thus enhancing hydrolysis performance relative to that of a coaxial probe. Effective on-line digestion was accomplished in ∼12 s. The probe should be of general utility for HDX-MS studies of amyloid fibrils and other protein aggregates.
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Authors
Maolian Chen, Kelsey D. Cook, Indu Kheterpal, Ronald Wetzel,